By Wan Azlina Ahmad, Wan Yunus Wan Ahmad, Zainul Akmar Zakaria, Nur Zulaikha Yusof
Environmental matters concerning non-stop use of artificial dyes observed a revival within the call for for normal dyes as typical dyes express larger biodegradability and customarily have a better compatibility with the surroundings. in spite of the fact that, one of many boundaries at the use of common dyes or pigments is the low extraction yield components (a few grams of pigment according to kg of dried uncooked material). as a result, the exploitation of different organic assets reminiscent of fungi, micro organism and mobile cultures bargains fascinating replacement. Microbial pigments resembling from bacterial origins provide the virtue when it comes to construction in comparison to pigments extracted from greens or animals, because of its easy telephone and quickly culturing method. This publication bargains attention-grabbing perception into preliminary works performed to illustrate the aptitude use of bacterial pigment as colorant for numerous applications.
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Extra resources for Application of Bacterial Pigments as Colorant: The Malaysian Perspective
2002, since the color shift may pass unnoticed when the KOH solution is poured directly over a thin colony on an agar plate, it is strongly recommended that the test be performed on a small mass of bacterial cells collected with a loop and deposited on a glass slide placed on a white background. Another similar mass of bacteria on which no KOH is poured may be used as a control. The color changed induced by KOH is not absolutely specific for the flexirubin-type of pigment (Fautz and Reichenbach 1980), but it is still helpful when combined with the results of other tests.
Violaceum when grown in SCB, SPW, M and BS (supplemented with 10% (v/v) L-tryptophan) (Fig. 11). After 24 h of incubation, the original pale-yellow appearance of SCB and green color of SPW turned to dark purple due to the formation of pigment. Adequate contents of cellulose, hemicellulose and lignin in SCB acted as a good C source as well as a useful adhesion site for bacterial growth and pigment production. However, this process only proceeds in a strictly aerobic condition as demonstrated by the impediment of pigment production in anaerobic condition (SCB5).
The solution was filtered (Whatman, UK) to remove insoluble materials 48 3 Optimization of Pigment Production Fig. 4 Growth profile of S. 3 kPa for 15 min (HVE-50, Hirayama). 4 shows the growth profile of S. marcescens in NB. For this growth profile, lag phase was not visible as exponential growing culture was used to inoculate the medium causing cells to commence growth immediately. The log phase lasted for about 8 h after inoculation into fresh medium. After 8 h the growth was slow and the population reached the stationary phase.
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