Biophysics for Therapeutic Protein Development by Linda O. Narhi (auth.), Linda O. Narhi (eds.)

By Linda O. Narhi (auth.), Linda O. Narhi (eds.)

This ebook can be utilized to supply perception into this significant software of biophysics in case you are making plans a occupation in protein healing improvement, and for these open air this zone who're drawn to realizing it larger. The preliminary chapters describe the underlying concept, and strengths and weaknesses of different ideas usual in the course of healing improvement. nearly all of the chapters talk about the purposes of those thoughts, together with case experiences, around the product lifecycle from early discovery, the place the point of interest is on opting for goals, and screening for power drug product applicants, via expression and purification, huge scale construction, formula improvement, lot-to-lot comparison reviews, and advertisement aid together with investigations.

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This technique can also be used to ­follow changes in the distance between parts of the same polypeptide with changes in solution condition, interactions with other proteins, etc. Most applications of FRET utilize extrinsic fluorophores, ones that involve cofactors as in the process of photosynthesis or more commonly are covalently attached to a protein via chemical means. These have found widespread applications in labeling of cellular proteins used in studies of cellular trafficking or in vivo protein–protein interactions (Pollok and Heim 1999; Miyawaki 2011; Jares-Erijman and Jovin 2003) and in studies of single molecules (Ferreon et al.

Biotechnol Genet Eng Rev 18:301–327 Feng BY, Shelat A, Doman TN, Guy RK, Shoichet BK (2005) High-throughput assays for promiscuous inhibitors. Nat Chem Biol 1(3):146–148 Fowler A, Swift D, Longman E et al (2002) An evaluation of fluorescence polarization and lifetime discriminated polarization for high throughput screening of serine/threonine kinases. Anal Biochem 308(2):223–231 2 High-Throughput Biophysical Approaches to Therapeutic Protein Development 29 Goldberg DS, Bishop SM, Shah AU, Sathish HA (2010) Formulation development of therapeutic monoclonal antibodies using high-throughput fluorescence and static light scattering techniques: role of conformational and colloidal stability.

J Sol–Gel Sci Technol 38(3):241–244 Schiffer CA, Dotsch V (1996) The role of protein-solvent interactions in protein unfolding. Curr Opin Biotechnol 7(4):428–432 2 High-Throughput Biophysical Approaches to Therapeutic Protein Development 31 Schmidt R (2010) Dynamic light scattering for protein characterization. Encyclopedia of Analytical Chemistry, 20–24 Sellick CA, Hansen R, Jarvis RM et al (2010) Rapid monitoring of recombinant antibody production by mammalian cell cultures using Fourier transform infrared spectroscopy and chemometrics.

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